Separation science is a core discipline of analytical chemistry. This course comprises the fundamentals, theory and practice of modern liquid chromatography, gas chromatography, and capillary electrophoresis, including common instrumentation and detection principles. Parameters to describe and predict separation performance are introduced. Various additional aspects such as chromatographic and electrophoretic modes, gradient elution, detector noise filtering, two-dimensional gas chromatography, and polymer analysis are treated. Lectures cover the various subjects, which are further elaborated in exercise tutorials. As part of the course, students will study and present a recent scientific paper on separation science.
About the lecturers
Govert Somsen is professor in Biomolecular Analysis at the Vrije Universiteit Amsterdam. His group built an extensive expertise in detailed profiling of pharmaceutical and industrial proteins by advanced CE-MS and LC-MS techniques. In the course he will address LC retention mechanisms, sample preparation, detection and capillary electrophoresis.
Bob Pirok did his PhD at the University of Amsterdam and specialised in two-dimensional liquid chromatography. His is interested in the use of chromatographic modelling and Chemometrics to improve the use of 2D-LC. In Separation Sciences, Bob will treat the fundamentals of packed-column and open-tubular chromatography, dispersion, signal analysis, gradient elution and polymer separations.
Peter Schoenmakers is professor at the University of Amsterdam. His work focuses on analytical separations in general and on multi-dimensional liquid chromatography in particular. In this course, he will focus on (two-dimensional) gas chromatography and injection techniques.